Basigin (BSG) Antikörper

Antigen: Basigin (BSG)

Synonyme: wu:fb26a05, wu:fo98e12, 135/10, BcDNA:GH21853, bsg, gel, l(2)06243, l(2)k06338, l(2)k09030, l(2)k13638, ms(2)08318, Dmel\CG31605, CG31605, BSG, RPE7, M6, OK, 5F7, TCSF, CD147, EMMPRIN, Bsg, GB11183, HT-7, AI115436, AI325119, CE9, HT7, 5A11, Ox47R, basignin

Klonalität: Monoklonal (MEM-M6-1)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN93981

Produktbeschreibung

Weitere Bezeichnung: CD147

Immunogen: Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of thecDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg issecreted by transfectants as a dimer).

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MEM-M6-1

Beschreibung: CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN(extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derivedcollagenase-stimulatory factor) is an ubiquitously expressed cell surface protein withmultiple glycosylated forms. The highest level of CD147 expression is on metabolicallyactive cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignanttumour cells. CD147 has multiple functions, including facilitating of cell surface expression ofmonocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulationof integrin functions, it plays roles in cell development and activation, fetal development orretinal function.

Spezifität: The antibody MEM-M6/1 recognizes an epitope in the N-terminal Ig domain (D1) of CD147(Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on allleukocytes, red blood cells, platelets and endothelial cells, it is not expressed by restinglymphocytes.The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulatedperipheral blood T cells.

Synonyme: wu:fb26a05, wu:fo98e12, 135/10, BcDNA:GH21853, bsg, gel, l(2)06243, l(2)k06338, l(2)k09030, l(2)k13638, ms(2)08318, Dmel\CG31605, CG31605, BSG, RPE7, M6, OK, 5F7, TCSF, CD147, EMMPRIN, Bsg, GB11183, HT-7, AI115436, AI325119, CE9, HT7, 5A11, Ox47R, basignin

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 1 µg/ml. Application note: Non-reducing conditions. Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: testis

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Krebs, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Schneiderhan, Scheler, Holzmann et al.: "CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro models." in: Gut, Vol. 58, Issue 10, pp. 1391-8, 2009 (PubMed).