Blood Group Lewis A Antikörper

Antigen: Blood Group Lewis A

Klonalität: Monoklonal (7LE)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), ELISA

Produktnummer: ABIN93924

Produktbeschreibung

Immunogen: Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pureendocervical type according to the Fenoglio's classification).

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 7LE

Spezifität: The antibody 7LE recognizes Lewis a blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewis a may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.

Anwendungen

Applikationshinweise: Immunohistochemistry (paraffin sections) - Recommended dilution: 5 µg/ml. Positive control: colorectal adenocarcinoma. Application note: heat mediated antigen retrieval (sodium citrate), mAb incubation 1 hour /RT, detection DAB

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemistry staining of colorectal adenocarcinoma (paraffin-embedded sections) with anti-human Blood Group Lewis a (7LE)

Publikationen

Publikationen: Creuzot-Garcher, Guerzider, Assem et al.: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, 1999 (PubMed).