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Antikörper
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anti-alpha-Fetoprotein (AFP) Antikörper
alpha-Fetoprotein (AFP) Antikörper
| Antigen |
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| Synonyme |
FETA, HPAFP, AFP, MGC128267 |
| Klonalität |
Monoklonal (AFP-11) |
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Wirt
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Reaktivität
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Human (231), Maus (48), Ratte (Rattus) (34), Hund (30), Schwein (26), Rind (Kuh) (23), Kaninchen (20), Pferd (17), Huhn (5), Schaf (4), Affe (3), Fruchtfliege (Drosophila melanogaster) (1), Säugetiere (1), Zebrafisch (Brachydanio rerio) (1)
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Applikation
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 Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p)), ELISA, Radioimmunoassay (RIA)
Western Blot (WB) (96), Enzyme Immunoassay (EIA) (69), Immunfluoreszenz (IF) (68), Durchflusszytometrie (FACS) (65), ELISA (56), Immunhistochemie (Paraffinschnitte) (IHC (p)) (52), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (32), Immunpräzipitation (IP) (24), Immunhistochemie (IHC) (21), Radioimmunoassay (RIA) (19), ELISA (Fangantikörper) (5), ELISA (Detektionsantikörper) (5), Immunelektrophorese (IEP) (5), Lateral Flow Messung (LF) (5), Immunodiffusion (ID) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (4), Immunzytochemie (ICC) (3), Immunoelectron Microscopy (IEM) (2), Radiale Immunodiffusion (RID) (2), Konjugation (1), ELISA (Beschichtung) (1), Gel Diffusion (GD) (1), Indirektes ELISA (iELISA) (1)
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| Produktnummer |
ABIN93887 |
| Menge |
0.1 mg (1 mg/ml) |
| Preis |
160,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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alpha-Fetoprotein
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Immunogen
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purified alpha-Fetoprotein
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Format
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Purified
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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AFP-11
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Spezifität
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The antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetalantigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues.Elevated AFP concentrations in adult plasma may be an early marker of hepatocellularcarcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severecongenital defects of a fetus.
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Applikationshinweise
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Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: hepatocellular carcinoma. Application note: heat mediated antigen retrieval (sodium citrate buffer), mAb incubation 1hour / RT, detection DABELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
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Konzentration
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1 mg/ml
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Reinheit
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> 95% (by SDS-PAGE)
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Reinigung
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Purified from ascites by precipitation methods.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Lagerung
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Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.
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Forschungsgebiet
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Serum/Plasma Proteine
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Stefanovua, Horejsui, Kristofovua et al.: "Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes." in: Journal of immunological methods, Vol. 111, Issue 1, pp. 67-73, 1988 (PubMed).
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Alternativen zu Antigen "alpha-Fetoprotein (AFP)", Typ "Antikörper" finden
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Wirte
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Maus (168), Kaninchen (97), Ziege (15), Schaf (3), Huhn (2), Ente (2)
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Reaktivitäten
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Human (231), Maus (48), Ratte (Rattus) (34), Hund (30), Schwein (26), Rind (Kuh) (23), Kaninchen (20), Pferd (17), Huhn (5), Schaf (4), Affe (3), Fruchtfliege (Drosophila melanogaster) (1), Säugetiere (1), Zebrafisch (Brachydanio rerio) (1)
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Applikationen
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Western Blot (WB) (96), Enzyme Immunoassay (EIA) (69), Immunfluoreszenz (IF) (68), Durchflusszytometrie (FACS) (65), ELISA (56), Immunhistochemie (Paraffinschnitte) (IHC (p)) (52), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (32), Immunpräzipitation (IP) (24), Immunhistochemie (IHC) (21), Radioimmunoassay (RIA) (19), ELISA (Fangantikörper) (5), ELISA (Detektionsantikörper) (5), Immunelektrophorese (IEP) (5), Lateral Flow Messung (LF) (5), Immunodiffusion (ID) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (4), Immunzytochemie (ICC) (3), Immunoelectron Microscopy (IEM) (2), Radiale Immunodiffusion (RID) (2), Konjugation (1), ELISA (Beschichtung) (1), Gel Diffusion (GD) (1), Indirektes ELISA (iELISA) (1)
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Konjugate
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HRP (7), Biotin (6), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 647 (4), FITC (4), PE,Cy5.5 (4), Alexa Fluor 555 (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
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Epitope
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N-Term (4), a (2), C-Term (1), Center (1), b (1), c (1)
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