Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

CD16 Antikörper (APC)

CD16 Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal 3G8 APC
Produktnummer ABIN807922
  • Target Alle CD16 Antikörper anzeigen
    CD16
    Reaktivität
    • 143
    • 26
    • 21
    • 21
    • 21
    • 8
    • 8
    • 8
    • 8
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Non-Human Primate
    Wirt
    • 127
    • 29
    • 14
    • 1
    Maus
    Klonalität
    • 143
    • 26
    • 1
    Monoklonal
    Konjugat
    • 59
    • 19
    • 13
    • 13
    • 8
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD16 Antikörper ist konjugiert mit APC
    Applikation
    • 136
    • 31
    • 31
    • 26
    • 24
    • 23
    • 22
    • 20
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Spezifität
    The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils). Regarding CD16-158V/F polymorphism, the antibody 3G8 detects both 158V and 158F allotype on natural NK cells.
    Kreuzreaktivität (Details)
    Human, Non-Human Primates
    Aufreinigung
    Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Human neutrophils
    Klon
    3G8
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product CD16 Primärantikörper
  • Applikationshinweise
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    Kommentare

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    No reconstitution is necessary.
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Avoid prolonged exposure to light.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Burt, Plitas, Zhao, Bamboat, Nguyen, Dupont, DeMatteo: "The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 3, pp. 1789-96, (2009) (PubMed).

    Choi, Wang, Peterson, Letvin, Reimann: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." in: Immunology, Vol. 124, Issue 2, pp. 215-22, (2008) (PubMed).

    Congy-Jolivet, Bolzec, Ternant, Ohresser, Watier, Thibault: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, (2008) (PubMed).

    Komano, Nanki, Hayashida, Taniguchi, Miyasaka: "Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts." in: Arthritis research & therapy, Vol. 8, Issue 5, pp. R152, (2007) (PubMed).

    Wijngaarden, van Roon, van de Winkel, Bijlsma, Lafeber: "Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment." in: Rheumatology (Oxford, England), Vol. 44, Issue 6, pp. 729-34, (2005) (PubMed).

    Metes, Ernst, Chambers, Sulica, Herberman, Morel: "Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene." in: Blood, Vol. 91, Issue 7, pp. 2369-80, (1998) (PubMed).

  • Target
    CD16
    Andere Bezeichnung
    CD16 (CD16 Produkte)
    Synonyme
    Fcgr3 antikoerper, Fcgr3a antikoerper, CD16 antikoerper, FCGR3 antikoerper, FCGR3@ antikoerper, CD16b antikoerper, FCG3 antikoerper, FCR-10 antikoerper, FCRIII antikoerper, FCRIIIb antikoerper, FcRIII antikoerper, Fc fragment of IgG, low affinity IIa, receptor antikoerper, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antikoerper, Fc fragment of IgG receptor IIIb antikoerper, Fc fragment of IgG receptor IIIa antikoerper, Fc receptor, IgG, low affinity III antikoerper, Fc-gamma RIII antikoerper, Fcgr2a antikoerper, FCGR3A antikoerper, FCGR3B antikoerper, Fcgr3 antikoerper, FCGR3 antikoerper
    Hintergrund
    CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Sie sind hier:
Kundenservice