GABRB3 Antikörper (pSer408, pSer409)

Produktnummer ABIN784601

Der Kaninchen Polyklonal anti-GABRB3 Antikörper wird verwendet zum Nachweis von GABRB3 in Proben von Human, Ratte, Maus, Rind (Kuh), Hund, Zebrafisch (Danio rerio), Huhn, Non-Human Primate und Xenopus laevis. Er wurde validiert für WB.

Kurzübersicht für GABRB3 Antikörper (pSer408, pSer409) (ABIN784601)

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Hund, Zebrafisch (Danio rerio), Huhn, Non-Human Primate, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABRB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GABRB3 Antikörper

Bindungsspezifität: pSer408, pSer409

Spezifität: The antibody has been directly tested for reactivity in Western blots with rat tissue. Specific for the ~53k GABAA receptor beta-3 subunit phosphorylated at Ser408/409. Immunolabeling of the GABAA band is completely blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, non-Human primates, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the di-phospho- Ser408/409 of the Rat GABAA receptor, beta-3 subunit.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C.

Details zu GABRB3

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Andere Bezeichnung: GABRB3

Hintergrund: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six Alpha's, four Beta's and four Gamma's, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for Alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a Gamma-subunit is required for benzodiazepine modulation. Phosphorylation of Serine 408 and Serine 409 within the Beta-3 subunit have been shown to be critical for the functional modulation of Beta-3 containing recombinant receptors (Brandon et al., 2000).Synonyms: GABA A receptor subunit beta-3, GABRB-3, Gamma-aminobutyric acid receptor subunit beta-3

Gen-ID: 24922

NCBI Accession: NP_058761

Pathways: Sensory Perception of Sound