Adenovirus 5 E1A Antikörper

Antigen: Adenovirus 5 E1A

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Bakterien, Virus

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN762941

Produktbeschreibung

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human Adenovirus 5 E1A

Format: Lyophilized

Wiederherstellung: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Synonym: Adenovirus early E1A protein, Control protein E1A, E1A, Early E1A protein, Human adenovirus type 5, Human adenovirus C, pfam02703.

Molekulargewicht: 32kDa

Kommentare:

The early region (E1) of the adenovirus genome

responsible for transforming activity

is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.

Anwendungen

Applikationshinweise: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Konzentration: 1mg/1ml

Reinigung: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Lagerung: Ship at room temperature. Store at -20°C

Beschränkungen: Nur für Forschungszwecke einsetzbar