LATS2 Antikörper (pSer83)

Produktnummer ABIN753568

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LATS2 in ELISA, WB, IHC (p), IF (cc), IF (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.Es wurde in 1 Publikation zitiert.

Kurzübersicht für LATS2 Antikörper (pSer83) (ABIN753568)

Target: LATS2 (LATS, Large Tumor Suppressor, Homolog 2 (Drosophila) (LATS2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LATS2 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-LATS2 Antikörper

Bindungsspezifität: pSer83

Spezifität: This phosphorylation site is homologous to that of Ser82 in Mouse and Ser83 Rat.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human LATS2 around the phosphorylation site of Ser83

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-LATS2 Antikörper (ABIN753568)

Zhang, Wang, Xie, Chen, Sinkemani, Yu, Wang, Mao, Wu: "Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence." in: Oncotarget, Vol. 9, Issue 2, pp. 2175-2192, (2018) (PubMed).

Details zu LATS2

Target: LATS2 (LATS, Large Tumor Suppressor, Homolog 2 (Drosophila) (LATS2))

Andere Bezeichnung: LATS2

Hintergrund:

Synonyms: KPM, Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Warts-like kinase, LATS2

Background: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.

Gen-ID: 26524

UniProt: Q9NRM7