PRKCA beta 2 Antikörper (pThr638, pThr641)

Produktnummer ABIN744758

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-PRKCA beta 2 Antikörper wird verwendet zum Nachweis von PRKCA beta 2 in Proben von Human, Hund, Maus und Ratte. Er wurde validiert für WB, IF (cc), IF (p), ELISA, IHC (fro) und IHC (p).

Kurzübersicht für PRKCA beta 2 Antikörper (pThr638, pThr641) (ABIN744758)

Target: PRKCA beta 2 (Protein Kinase C alpha beta 2 (PRKCA beta 2))

Reaktivität: Human, Hund, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKCA beta 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PRKCA beta 2 Antikörper

Bindungsspezifität: pThr638, pThr641

Kreuzreaktivität: Hund, Maus, Ratte

Homologie: Human,Cow,Horse,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PKC alpha/beta II around the phosphorylation site of Thr638/641

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-PRKCA beta 2 Antikörper (ABIN744758)

Edens, Dilsaver, Levy: "PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells." in: Molecular biology of the cell, Vol. 28, Issue 10, pp. 1389-1399, (2017) (PubMed).

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

Edens, Levy: "cPKC regulates interphase nuclear size during Xenopus development." in: The Journal of cell biology, Vol. 206, Issue 4, pp. 473-83, (2014) (PubMed).

Details zu PRKCA beta 2

Target: PRKCA beta 2 (Protein Kinase C alpha beta 2 (PRKCA beta 2))

Hintergrund:

Synonyms: AAG6, PKCA, PRKACA, PKC-alpha, Protein kinase C alpha type, PKC-A, PRKCA

Background: Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

Gen-ID: 5578

UniProt: P17252