Nestin (NES) Antikörper

Antigen: Nestin (NES)

Synonyme: FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus), Maus

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN723530

Produktbeschreibung

Weitere Bezeichnung: Nestin

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from rat Nestin

Format: Lyophilized

Wiederherstellung: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Synonym: ESTM 46, FLJ 21841, FLJ21841, Intermediate filament protein, Nbla00170, Nes

Molekulargewicht: 178kDa

Kommentare:

Neural Stem Cell Marker Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells

glioma cells

and tumor endothelial cells in the mammalian CNS. Furthermore

it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Anwendungen

Applikationshinweise: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Konzentration: 1mg/1ml

Reinigung: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Lagerung: Ship at room temperature. Store at -20°C

Forschungsgebiet: Angiogenese, Abstammungsmarker

Beschränkungen: Nur für Forschungszwecke einsetzbar