Sclerostin (SOST) Antikörper

Antigen: Sclerostin (SOST)

Synonyme: VBCH, 5430411E23Rik, SOST, LOC100313724

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN715586

Produktbeschreibung

Weitere Bezeichnung: Sclerostin

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human Sclerostin

Format: Lyophilized

Wiederherstellung: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Synonym: BEER, Cortical hyperostosis with syndactyly, Sclerosteosis, Sclerostin, SOST, SOST_HUMAN, VBCH.

Molekulargewicht: 24-28kDa

Kommentare:

Negative regulator of bone growth.Sclerostin (SOST) is a bone morphogenetic protein (BMP) antagonist

leading to the activation of BMP signaling. It negatively regulates the formation of bone by repressing the differentiation and/or function of osteoblasts induced by BMPs. It has been shown that Sclerostin binds BMP-5

-6

and -7 with high affinity and BMP-2 and -4 with low affinity. The noggin-sclerostin protein complex represents a novel mechanism for the fine-tuning of BMP activity in bone homeostasis. Evidence is accumulating that one of the important mechanisms of bone regulation by sclerostin is the modulation of Wnt/Beta-catenin signaling. Sclerostin also rapidly activated ERK-1/2 MAPK signaling

indicating the involvement of additional signaling pathways.

Anwendungen

Applikationshinweise: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Konzentration: 1mg/1ml

Reinigung: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Lagerung: Ship at room temperature. Store at -20°C

Forschungsgebiet: Stammzellen, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar