CYP27B1 Antikörper (C-Term)

Produktnummer ABIN652999

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CYP27B1 in WB, FACS und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Ratte. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für CYP27B1 Antikörper (C-Term) (ABIN652999)

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CYP27B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB16978

Produktdetails anti-CYP27B1 Antikörper

Bindungsspezifität: AA 482-508, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human CYP27B1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:500. WB: 1:2000. IHC-P: 1:10~50. FC: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-CYP27B1 Antikörper (ABIN652999)

Chiang, Yeh, Huang, Yeh, S Pang, Hsu, Chen, Kuo, Kittaka, Chen, Juang: "25(OH)D Is Effective to Repress Human Cholangiocarcinoma Cell Growth through the Conversion of 25(OH)D to 1α,25(OH)₂D₃." in: International journal of molecular sciences, Vol. 17, Issue 8, (2016) (PubMed).

Schlosser, Carroll, Soler, Pasquini, Mulligan: "Reduced sinonasal levels of 1?-hydroxylase are associated with worse quality of life in chronic rhinosinusitis with nasal polyps." in: International forum of allergy & rhinology, (2015) (PubMed).

Mandell, Seedorf, Ryan, Gien, Cramer, Abman: "Antenatal endotoxin disrupts lung vitamin D receptor and 25-hydroxyvitamin D 1?-hydroxylase expression in the developing rat." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 309, Issue 9, pp. L1018-26, (2015) (PubMed).

Mulligan, Nagel, OConnell, Wentzel, Atkinson, Schlosser: "Cigarette smoke exposure is associated with vitamin D3 deficiencies in patients with chronic rhinosinusitis." in: The Journal of allergy and clinical immunology, Vol. 134, Issue 2, pp. 342-349.e1, (2014) (PubMed).

Details zu CYP27B1

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Andere Bezeichnung: CYP27B1

Hintergrund: CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Molekulargewicht: 56504

Gen-ID: 1594

NCBI Accession: NP_000776

UniProt: O15528

Pathways: Metabolism of Steroid Hormones and Vitamin D