F-Actin Antikörper

Antigen: F-Actin

Wirt: Maus

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN640417

Produktbeschreibung

Produktmerkmale: Mouse Anti-Human F-Actin

Wiederherstellung: Antibody is supplied as 500 mg purified IgM in phosphate-buffered saline pH 7.4, with 0.09% sodium azide Its final concentration is 1.0 mg/mL.

Isotyp: IgM  (Passende Sekundärantikörper)

Beschreibung: Actin is a globular, 42-47 kDa, structural protein found in eukaryotic cells. Actin has two main functions in cells. In all cells it forms the thinnest part of the cytoskeleton, which allows motility, while in muscle cells it also associates with myosin proteins to form the contractile apparatus. Individual subunits of actin are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin. Because it interacts with a large number of other proteins, actin is one of the most highly conserved throughout evolution. Mammals have at least six actin isoforms coded by separate genes, which are divided into three classes (alpha, beta and gamma) according to their isoelectric point. Alpha actins are generally found in muscle, whereas beta and gamma isoforms are prominent in non-muscle cells. Although there are small differences in sequence and properties between the isoforms, all actins assemble into microfilaments and are essentially identical in the majority of tests performed in vitro.

Spezifität: This antibody recognizes human filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types. Cross-reacts with rabbit F-actin

Anwendungen

Reinigung: Purified

Lagerung: If not intended for use within a month, store at -20 ° C,. Otherwise, keep it at 4 ° C and minimize freezing and thawing when use. Should this product contain a precipitate we recommend microcentrifugation before use. Stable for 18 months. Please avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar