P2RX7 Antikörper (N-Term)

Produktnummer ABIN571021

Dieser Ziege Polyklonal Antikörper detektiert spezifisch P2RX7 in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für P2RX7 Antikörper (N-Term) (ABIN571021)

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser P2RX7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-P2RX7 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: P2RX7 / P2X7 receptor

Sequenz: YETNKVTRIQ SMNY

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence YETNKVTRIQSMNY-C, from the N Terminus of the protein sequence according to NP_002553.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Brain Cortex shows membranous staining of cell bodies and processes. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 37 kDa and 70 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6 kDa according to NP_002553.2). Recommended concentration: 1-3 μg/mL. Primary incubation was an 1 hour. An additional band of unknown identity

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: This antibody has been successfully used in IF on Mouse: Thaler R et al. (2015) PMID: 25491310.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu P2RX7

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Andere Bezeichnung: P2RX7

Hintergrund: P2RX7, purinergic receptor P2X, ligand-gated ion channel, 7, MGC20089, P2X7, ATP receptor, P2X purinoceptor 7, P2X7 receptor, P2Z receptor, purinergic receptor P2X7

Gen-ID: 5027, 18439, 29665

NCBI Accession: NP_002553

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis