TSPO Antikörper (C-Term)

Produktnummer ABIN570960

Zitiert in 1 Publikation. Der Ziege Polyklonal anti-TSPO Antikörper (ABIN570960) detektiert spezifisch TSPO in WB, IHC, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für TSPO Antikörper (C-Term) (ABIN570960)

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TSPO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-TSPO Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PBR (mouse)

Sequenz: RDNSGRRGGS RLPE

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RDNSGRRGGSRLPE, from the C Terminus of the protein sequence according to NP_033905.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Mouse Kidney and Colon. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx.16-17 kDa band observed in Mouse Kidney lysates and in lysates of cell line NIH3T3 (calculated MW of 18.8 kDa according to NP_033905.3). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-TSPO Antikörper (ABIN570960)

Morgan, Cheepala, Wang, Neale, Adachi, Nachagari, Leggas, Zhao, Boyd, Venkataramanan, Schuetz: "Deregulated hepatic metabolism exacerbates impaired testosterone production in Mrp4-deficient mice." in: The Journal of biological chemistry, Vol. 287, Issue 18, pp. 14456-66, (2012) (PubMed).

Details zu TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Andere Bezeichnung: Tspo

Hintergrund: PBR, Tspo, translocator protein, Bzrp, IBP, benzodiazepine receptor, peripheral, isoquinoline-binding protein, peripheral benzodiazepine receptor

Gen-ID: 12257, 24230

NCBI Accession: NP_033905

Pathways: Cellular Response to Molecule of Bacterial Origin