Subtilisin Antikörper

Produktnummer ABIN568427

Der Kaninchen Polyklonal anti-Subtilisin Antikörper wird verwendet zum Nachweis von Subtilisin in Proben von Bacillus. Er wurde validiert für IF, WB, DB, EIA, ID, IP und RIA.

Kurzübersicht für Subtilisin Antikörper (ABIN568427)

Target: Subtilisin

Reaktivität: Bacillus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Produktdetails

Spezifität: Subtilisin from Bacillus subtilis. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Aufreinigung: Solid Phase Affinity Chromatography

Immunogen: Subtilisin isolated and purified from Bacillus subtilis. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 0.5 mL of sterile distilled water

Konzentration: 2.0 mg/mL

Buffer: PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Antigendetails

Target: Subtilisin

Hintergrund: Subtilisin (serine endopeptidase) is a non-specific protease (a protein-digesting enzyme) initially obtained from Bacillus subtilis. Subtilisins belong to subtilases, a group of serine proteases that initiate the nucleophilic attack on the peptide (amide) bond through a serine residue at the active site. They are physically and chemically well-characterized enzymes. Subtilisins typically have molecular weights of about 20,000 to 45,000 dalton. They can be obtained from soil bacteria, for example, Bacillus amyloliquefaciens. Subtilisins are secreted in large amounts from many Bacillus species. Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.Synonyms: Subtilisin E, apr, aprA, aprE, sprE

Gen-ID: 939313

NCBI Accession: NP_388911

UniProt: P04189