FITC Antikörper

Produktnummer ABIN567764

Der Maus Monoklonal anti-FITC Antikörper wird verwendet zum Nachweis von FITC. Er wurde validiert für WB, IHC (fro), IF und EIA.

Kurzübersicht für FITC Antikörper (ABIN567764)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FITC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Klon: NI 239

Produktdetails anti-FITC Antikörper

Aufreinigung: Purified

Immunogen: Highly purified Fluorescein Isothiocyanate isomer 1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 0.5 mL sterile distilled water

Konzentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 without foreign proteins.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated thawing and freezing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Details zu FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Andere Bezeichnung: FITC

Substanzklasse: Chemical

Hintergrund: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate