EBF1 Antikörper (Internal Region)

Produktnummer ABIN499164

Der Ziege Polyklonal anti-EBF1 Antikörper (ABIN499164) detektiert spezifisch EBF1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für EBF1 Antikörper (Internal Region) (ABIN499164)

Target: EBF1 (Early B-Cell Factor 1 (EBF1))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EBF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-EBF1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-HNQLPALANT SV

Spezifität: This antibody detects EBF1 / COE1 (Internal region). It is expected not to cross-react with EBF2, EBF3 and EBF4.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, ratSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_076870.1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: limit dilution 1: 64000. Western blot: 0.3-0.1 μg/mL, approx.75 kDa band observed in Human Adipose lysates. Anonymous customer used this product successfully in IP from induced 3T3-L1 expressingFLAG-EBF1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Details zu EBF1

Target: EBF1 (Early B-Cell Factor 1 (EBF1))

Andere Bezeichnung: EBF1 / COE1

Hintergrund: EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.Synonyms: EBF, Early B-cell factor, OE-1, Transcription factor COE1

Gen-ID: 1879

NCBI Accession: NP_076870

UniProt: Q9UH73