EIF2B4 Antikörper (Internal Region)

Produktnummer ABIN499136

Der Ziege Polyklonal anti-EIF2B4 Antikörper wird verwendet zum Nachweis von EIF2B4 in Proben von Human, Maus, Ratte, Rind (Kuh), Kaninchen und Hund. Er wurde validiert für WB und EIA.

Kurzübersicht für EIF2B4 Antikörper (Internal Region) (ABIN499136)

Target: EIF2B4 (Eukaryotic Translation Initiation Factor 2B, Subunit 4 Delta, 67kDa (EIF2B4))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EIF2B4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-EIF2B4 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-RKDYGSKVSL FSH

Spezifität: Recognizes EIF2B4. This antibody is expected to recognize all reported isoforms (NP_751945.2, NP_001029288.1 and NP_056451.3).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine, Rabbit.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to (NP_751945.2, NP_001029288.1 and NP_056451.3). Genename: EIF2B4

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.3-1 μg/mL. This antibody detects a approx. 60 kDa band in lysates of cell lineK562. An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu EIF2B4

Target: EIF2B4 (Eukaryotic Translation Initiation Factor 2B, Subunit 4 Delta, 67kDa (EIF2B4))

Andere Bezeichnung: EIF2B4 / EIF2BD

Hintergrund: EIF2B4, also called eIF2B delta is part of the eIF2B complex, which contains 5 subunits. It catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP (referenced from swissprot).Synonyms: Translation initiation factor eIF-2B subunit delta, eIF-2B GDP-GTP exchange factor subunit delta

Gen-ID: 8890

NCBI Accession: NP_001029288

UniProt: Q9UI10

Pathways: Methionine Biosynthetic Process