ZC3HC1 Antikörper

Produktnummer ABIN498264

Der Kaninchen Polyklonal anti-ZC3HC1 Antikörper (ABIN498264) detektiert spezifisch ZC3HC1 in WB und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für ZC3HC1 Antikörper (ABIN498264)

Target: ZC3HC1 (Zinc Finger, C3HC-Type Containing 1 (ZC3HC1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZC3HC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-ZC3HC1 Antikörper

Spezifität: This antibody detects endogenous levels of NIPA protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity-Chromatography using epitope-specific immunogen

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH ~7.2 with 0.05 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ZC3HC1

Target: ZC3HC1 (Zinc Finger, C3HC-Type Containing 1 (ZC3HC1))

Andere Bezeichnung: ZC3HC1

Hintergrund: Entry into mitosis is essentially driven by cyclin B1, which is located in the cytoplasm throughout interphase, but accumulates in the nucleus just before mitosis occurs. Nuclear interaction partner of ALK (NIPA) plays a critical role in cyclin B1 regulation. NIPA is normally phosphorylated during G2 and M phases, resulting in an accumulation of cyclin B1. When NIPA sheds its attached phosphate, it binds to SCF to form the SCFNIPA complex, a member of the E3 ubiquitin ligases, which ubiquitinates cyclin B1, thereby targeting it to the proteosome for degradation. Therefore, the accumulation of cyclin B1 is due to the inability of phosphorylated NIPA to bind to the molecule SCF, thereby preventing the degradation of cyclin B1. An absence of NIPA causes cyclin B1 to accumulate abnormally, leading to premature mitotic entry, loss of checkpoint control and genomic instability, which are all associated with cancer. The phosphorylated form of NIPA may also be involved in apoptotic signaling pathways.Synonyms: HSPC216, NIPA, Nuclear-interacting partner of anaplastic lymphoma kinase (ALK), Zinc finger C3HC-type protein 1

Molekulargewicht: approx. 55 kDa

Gen-ID: 51530

NCBI Accession: NP_057562

UniProt: Q86WB0