NDUFS1 Antikörper (Internal Region)

Produktnummer ABIN498087

Der Ziege Polyklonal anti-NDUFS1 Antikörper (ABIN498087) detektiert spezifisch NDUFS1 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Rind (Kuh), Kaninchen und Hund.

Kurzübersicht für NDUFS1 Antikörper (Internal Region) (ABIN498087)

Target: NDUFS1 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase) (NDUFS1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Kaninchen, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NDUFS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-NDUFS1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-TEKSATYVNT EGR

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine and RabbitSpecies reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_004997.4. Genename: NDUFS1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 80 kDa in Human Heart and Mouse Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human, Mouse. Expected from sequence similarity: Rat, Canine (Dog), Bovine and RabbitAdd. Information: Calculated Molecular Weight: 79.0 kDa according to NP_004997.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NDUFS1

Target: NDUFS1 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase) (NDUFS1))

Andere Bezeichnung: NDUFS1

Hintergrund: NDUFS1 belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. Ndufs1 has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in Ndufs1 gene are associated with complex I deficiency.Synonyms: CI-75kD, Complex I-75kD, NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gen-ID: 4719

NCBI Accession: NP_004997

UniProt: P28331