Toc75 Antikörper (Chloroplastic, Domain 3)

Produktnummer ABIN5684021

Der Kaninchen Polyklonal anti-Toc75 Antikörper wird verwendet zum Nachweis von Toc75 in Proben von Pea, Synechococcus sp., Synechocystis, Thermosynechococcus, Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomytrium Patens, Spinach und Thellungiella salsuginea. Er wurde validiert für FACS, IL und WB.

Kurzübersicht für Toc75 Antikörper (Chloroplastic, Domain 3) (ABIN5684021)

Target: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Reaktivität: Pea, Synechococcus sp., Synechocystis, Thermosynechococcus, Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomytrium Patens, Spinach, Thellungiella salsuginea

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Toc75 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)

Produktdetails anti-Toc75 Antikörper

Bindungsspezifität: Chloroplastic, Domain 3

Verwendungszweck: Anti-Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3

Kreuzreaktivität (Details): Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Aegilops tauschii,Ananas comosus,Anthurium amnicola,Capsicum annuum,Catalpa bungei,Cicer arietinum,Cucumis melo,Glycine soja,Gossypium arboreum,Medicago truncatula,Morus notabilis,Nelumbo nucifera,Nicotiana sylvestris,Noccaea caerulescens,Populus trichocarpa,Ricinus communis,Sorghum bicolor,Theobroma cacao,Zea mays,Zostera marina,Vigna radiata,Vitis vinifera

Aufreinigung: Serum

Immunogen: Pisum sativum TOC75, Predicted POTRA Domain #3, Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. UniProt: Q43715

Anwendungsinformationen

Applikationshinweise: 1 : 500 (Flow cyt), 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)

Kommentare:

antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Details zu Toc75

Target: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Hintergrund: OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]

Molekulargewicht: 88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

UniProt: Q43715