TAF4 Antikörper (Middle Region)

Produktnummer ABIN487064

Der Kaninchen Polyklonal anti-TAF4 Antikörper wird verwendet zum Nachweis von TAF4 in Proben von Human, Maus, Rind (Kuh), Hund, Meerschweinchen, Pferd und Kaninchen. Er wurde validiert für WB und ChIP.

Kurzübersicht für TAF4 Antikörper (Middle Region) (ABIN487064)

Target: TAF4 (TAF4 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 135kDa (TAF4))

Reaktivität: Human, Maus, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TAF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-TAF4 Antikörper

Bindungsspezifität: Middle Region

Sequenz: EQASDVRAQL KFFEQLDQIE KQRKDEQERE ILMRAAKSRS RQEDPEQLRL

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 78%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against TAF4. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TAF4

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1085 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu TAF4

Target: TAF4 (TAF4 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 135kDa (TAF4))

Andere Bezeichnung: TAF4

Hintergrund: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF4 is one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FLJ41943, TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
Protein Interaction Partner: SOX6, SUMO2, UBC, FBXO6, MED26, HIST3H3, TBP, GTF2F2, JUN, SP1, HTT, AHR, TAF9B, TRRAP, TAF13, TAF1L, TAF3, TAF10, TAF9, TAF7, TAF6, TAF1, TAF12, ELAVL1, TAF8, ATF7, TCF12, CREB1, TBPL1, GTF2A1, TAF11, TAF5, TAF2, CBX5, CBX3, GTF2A2, CREM, SAP130, SUPT3H,
Protein Size: 1085

Molekulargewicht: 110 kDa

Gen-ID: 6874

NCBI Accession: NM_003185, NP_003176

UniProt: O00268