RETNLB Antikörper

Produktnummer ABIN465494

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-RETNLB Antikörper wird verwendet zum Nachweis von RETNLB in Proben von Maus. Er wurde validiert für WB und ELISA.

Kurzübersicht für RETNLB Antikörper (ABIN465494)

Target: RETNLB (Resistin Like beta (RETNLB))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RETNLB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-RETNLB Antikörper

Spezifität: Mouse RELMbeta

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine RELMbeta.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100  mM Citric Acid was used. Information and photos are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMbeta (LS-C104626) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMbeta. Western Blot: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMbeta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Referenzen für anti-RETNLB Antikörper (ABIN465494)

Varyani, Löser, Filbey, Harcus, Drurey, Poveda, Rasid, White, Smyth, Gerbe, Jay, Maizels: "The IL-25-dependent tuft cell circuit driven by intestinal helminths requires macrophage migration inhibitory factor (MIF)." in: Mucosal immunology, (2022) (PubMed).

Gerbe, Sidot, Smyth, Ohmoto, Matsumoto, Dardalhon, Cesses, Garnier, Pouzolles, Brulin, Bruschi, Harcus, Zimmermann, Taylor, Maizels, Jay: "Intestinal epithelial tuft cells initiate type 2 mucosal immunity to helminth parasites." in: Nature, Vol. 529, Issue 7585, pp. 226-30, (2016) (PubMed).

Details zu RETNLB

Target: RETNLB (Resistin Like beta (RETNLB))

Andere Bezeichnung: FIZZ1 / RELM-Beta

Hintergrund: Name/Gene ID: RETNLB

Synonyms: RETNLB, CCRG, Fizz1, HXCP2, Found in inflammatory zone 1, Resistin like beta, Resistin-like beta, RELM-beta, RELMb, RELMbeta, RETNL2, FIZZ2, Xcp2

Gen-ID: 84666

UniProt: Q9BQ08

Pathways: Hormone Activity