CCL20 Antikörper

Produktnummer ABIN465457

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CCL20 in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CCL20 Antikörper (ABIN465457)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CCL20 Antikörper

Spezifität: Human MIP-3alpha

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIP-3alpha.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect Human MIP-3alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-3alpha (LS-C104612) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3alpha. Western Blot: To detect HumanMIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Andere Bezeichnung: CCL20 / MIP-3-Alpha

Hintergrund: Name/Gene ID: CCL20
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL20, Beta chemokine exodus-1, Beta-chemokine exodus-1, CKb4, Exodus-1, MIP-3a, MIP-3-alpha, LARC, ST38, SCYA20, C-C motif chemokine 20, CC chemokine LARC, Mip-3alpha, MIP3A, Small-inducible cytokine A20

Gen-ID: 6364

UniProt: P78556

Pathways: Phosphorylierungen bei SARS-CoV-2 Infektion