CCL5 Antikörper

Produktnummer ABIN465158

Der Maus Monoklonal anti-CCL5 Antikörper wird verwendet zum Nachweis von CCL5 in Proben von Human. Er wurde validiert für Neut.

Kurzübersicht für CCL5 Antikörper (ABIN465158)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CCL5 Antikörper ist unkonjugiert

Applikation: Neutralization (Neut)

Produktdetails anti-CCL5 Antikörper

Spezifität: Human RANTES

Aufreinigung: Protein A purified

Immunogen: E.coli derived recombinant Human RANTES.

Type of Immunogen: Cell extract

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: Neut (5 - 7 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/mL), a concentration of 3-5 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with biotinylated antigen affinity purified anti-human RANTES (LS-C104720) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Andere Bezeichnung: CCL5 / RANTES

Hintergrund: Name/Gene ID: CCL5
Family: Intercrine

Synonyms: CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228

Gen-ID: 6352

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration