Schaf anti-Maus IgG2a(1b), Allotype Specific Antikörper

Antigen: IgG2a(1b), Allotype Specific

Klonalität: Polyklonal

Wirt: Schaf

Reaktivität: Maus

Konjugat: Unkonjugiert

Applikation: ELISA, Immunhistochemie (IHC), Immunzytochemie (ICC)

Produktnummer: ABIN458534

Produktbeschreibung

Produktmerkmale: Purified IgG fraction of polyclonal sheep antiserum to mouse IgG2a(1b), allotype specific

Immunogen: Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Beschreibung: The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. Cross-reactivity The antiserum does not cross-react with any other component of the mouse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants.

Spezifität: The antiserum does not cross-react with any other component of the mouse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, expected chance of cross-reactivity will be small. Physicochemical characteristic IgG protein concentration 10 mg/ml. No foreign proteins added.

Anwendungen

Applikationshinweise: As unlabelled primary or secondary reagent for indirect detection of IgG2a(1b) at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. W hen applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:4.000. These data should be interpreted as general recommendations only. Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reinigung: The IgG (7S) fraction is isolated and purified from selected antisera and contains the of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Buffer: Purified hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline (pH7.2). Preservative: No preservative added, as it may interfere with the antibody activity.

Lagerung: Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days. The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Beschränkungen: Nur für Forschungszwecke einsetzbar