Ig kappa Light Chain (Free & Bound) Antikörper

Antigen: Ig kappa Light Chain (Free & Bound)

Klonalität: Polyklonal

Wirt: Schwein

Reaktivität: Human

Applikation: ELISA, Immunfluoreszenz (IF), Immunzytochemie (ICC)

Produktnummer: ABIN458243

Produktbeschreibung

Produktmerkmale: IgG fraction of polyclonal swine antiserum to free and bound human Ig kappa light chain

Weitere Bezeichnung: Ig kappa Light Chain (Free and Bound)

Immunogen: A pool of purified Bence Jones kappa proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Beschreibung: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants.

Spezifität: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail. Physicochemical characteristic IgG protein concentration is 10 mg/ml. No foreign proteins added.

Anwendungen

Applikationshinweise: s This IgG preparation is intended for use in precipitating (gel diffusion) and non-precipitating antibody-binding immunoassays (indirect immunofluorescence, enzyme immunoassays for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the kappa type as well as free light chains or Bence Jones proteins of the kappa type in serum or other body fluids., to prepare an insoluble immunoaffinity adsorbent or a solid phase catching antibody reagent by coupling to an artificial carrier, for labeling with a marker of the user’s own choice. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Working dilutions fro histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000 Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins.

Konzentration: Antibody titre: Precipitin titre not less than 1:32 when tested against normal human serum in agar immunodiffusion block titration.

Reinigung: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Buffer: Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (pH 7.2). Preservative: No preservative added, as it may interfere with the antibody activity.

Lagerung: Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days. The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen , and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Beschränkungen: Nur für Forschungszwecke einsetzbar