Immunoglobulin E (IgE) Antikörper (Biotin)

Antigen: Immunoglobulin E (IgE)

Klonalität: Monoklonal (BSW17-NBS01)

Wirt: Maus

Reaktivität: Human

Konjugat: Biotin

Applikation: ELISA, Western Blot (WB), Durchflusszytometrie (FACS), Funktionale Studien (Func)

Produktnummer: ABIN457504

Produktbeschreibung

Gen-ID: IGHE, chromosome 14q32.33

Immunogen: purified human myeloma IgE

Format: Affinity-purified

Isotyp: IgG1 kappa

Klon: BSW17-NBS01

Beschreibung: The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.

Spezifität: Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes

Sensitivität: Reactivity > 95%, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Anwendungen

Applikationshinweise: Sterile filtered solution, does not contain Na-azide. Recommended for functional cellular assays (mast cell / basophil activation).

Konzentration: 1mg/ml, Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Reinheit: > 85%, tested by analytical Gel Permeation Chromatography

Reinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B – rat monoclonal anti- mouse Ck

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Lagerung: Store undiluted at 4 °C. For long term storage, aliquot and store at -20°C or -80°C

Forschungsgebiet: Allergie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

product purity: gel permeation chromatography (Superose 12/HR)

ELISA binding to human IgE(SUS-11), Detection: Streptavidin-HRP