Actin Antikörper

Antigen: Actin

Synonyme: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Klonalität: Monoklonal (HHF35)

Wirt: Maus

Reaktivität: Human, Primaten, Maus, Ratte (Rattus), Hund, Katze, Kaninchen, Huhn

Applikation: Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p)), Radioimmunoassay (RIA)

Produktnummer: ABIN457424

Produktbeschreibung

Immunogen: SDS extracted protein fraction of human myocardium

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: HHF35

Beschreibung: Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to formfibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have beenidentified, that fall into three classes. Alpha actin is a structural component of the contractileapparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles inregulation of cell motility in other cell types. Specific subcellular structures such as as stressfibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.

Spezifität: The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin(42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells aswell as myoepithelial cells and pericytes of small vessels. It is a widely used marker ofmuscle and muscle-derived cells.

Synonyme: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: murine femoral muscle, murine heart. Negative control: HUVEC line. Application note: reducing conditions. Immunohistochemistry (paraffin sections). Application note: Antigen retrieval steps generally not required, but e. g. in case of arterialsmooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Krebs, Apoptose/Nekrose, Zytoskelett, Mikrofilamente, Extrazelluläre Matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar