Selectin L (SELL) Antikörper

Antigen: Selectin L (SELL)

Synonyme: TQ1, LAM1, LNHR, LSEL, CD62L, LAM-1, LYAM1, Leu-8, PLNHR, hLHRc, LECAM1, Lyam-1, Lnhr, Ly-22, Lyam1, Ly-m22, LECAM-1, AI528707, L-selectin

Klonalität: Monoklonal (DREG56)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Funktionale Studien (Func)

Produktnummer: ABIN457418

Produktbeschreibung

Weitere Bezeichnung: CD62L

Immunogen: PMA-activated human peripheral blood leukocytes

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: DREG56

Beschreibung: CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cellsurface of leukocytes and mediates their homing to inflammatory sites and peripheral lymphnodes by enabling rolling along the venular wall. CD62L is also involved inactivation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however,counteracts neutrophil rolling. CD62L has also signaling roles including enhance ofchemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1(P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to helpdistinguish naive T cells from effector/memory T cells.

Spezifität: The mouse monoclonal antibody DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cellsurface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells,that interacts with specific carbohydrates exposed on activated endothelial cells.HLDA V, WS Code S056

Anwendungen

Applikationshinweise: Flow Cytometry: Recommended dilution: 2 µg/ml. Application note: non-reducing conditionsImmunohistochemistry (frozen sections). Functional ApplicationParticularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymphnode HEV

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar