DLC1 Antikörper (Isoform 1, Isoform 3, N-Term)

Produktnummer ABIN452680

Dieser Ziege Polyklonal Antikörper detektiert spezifisch DLC1 in WB, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Schwein.

Kurzübersicht für DLC1 Antikörper (Isoform 1, Isoform 3, N-Term) (ABIN452680)

Target: DLC1 (Deleted in Liver Cancer 1 (DLC1))

Reaktivität: Human, Maus, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DLC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-DLC1 Antikörper

Bindungsspezifität: Isoform 1, Isoform 3, N-Term

Sequenz: SVAIRKRSWE EHC

Spezifität: This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3) but NOT isoform 2 (NP_006085.2).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Pig.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_872584.2, NP_079043.3. Genename: DLC1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Antibody Detection Limit Dilution: 1/2,000. Western Blot: Preliminary experiments gave an approx 28 kDa band in Human Liver andLung lysates as well as in different epithelial lung cell lines after 0.3 μg/mL antibodystaining. Please note that currently we cannot find an explanation in the literature for theband we observe given the calculated size of 171 kDa according to NP_872584.1 and54.8 kDa according to NP_079043.2 (isotope 3). The 28 kDa band was successfully blockedby incubation with the immunizing peptide. Immunohistochemistry: 5 μg/mL. In paraffin embedded Human Adrenal Gland (Medulla)shows strong reticulate staining in the cytoplasm of medular cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu DLC1

Target: DLC1 (Deleted in Liver Cancer 1 (DLC1))

Andere Bezeichnung: ARHGAP7 / DLC1

Hintergrund: DLC1 functions as a GTPase-activating protein specific for Rho and an activator of PLCD1 in vivo and induces morphological changes and detachment through cytoskeletal reorganization. DLC1 is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers.Synonyms: Deleted in liver cancer 1 protein, HP protein, KIAA1723, Rho GTPase-activating protein 7, Rho-type GTPase-activating protein 7, STARD12, START domain-containing protein 12, StAR-related lipid transfer protein 12

Gen-ID: 10395

NCBI Accession: NP_006085

UniProt: Q96QB1

Pathways: Tube Formation, Positive Regulation of Endopeptidase Activity