»
Antikörper
»
anti-alpha-Fetoprotein (AFP) Antikörper
alpha-Fetoprotein (AFP) Antikörper
| Antigen |
|
| Synonyme |
FETA, HPAFP, AFP, MGC128267 |
| Klonalität |
Monoklonal (AFP-Y1) |
|
Wirt
|
|
|
Reaktivität
|
Human (231), Maus (48), Ratte (Rattus) (34), Hund (30), Schwein (26), Rind (Kuh) (23), Kaninchen (20), Pferd (17), Huhn (5), Schaf (4), Affe (3), Fruchtfliege (Drosophila melanogaster) (1), Säugetiere (1), Zebrafisch (Brachydanio rerio) (1)
|
|
Applikation
|
 Alternativen Enzyme Immunoassay (EIA), ELISA (Fangantikörper), Immunpräzipitation (IP), Radioimmunoassay (RIA), Western Blot (WB)
Western Blot (WB) (95), Enzyme Immunoassay (EIA) (68), Immunfluoreszenz (IF) (68), Durchflusszytometrie (FACS) (65), ELISA (57), Immunhistochemie (Paraffinschnitte) (IHC (p)) (53), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (32), Immunpräzipitation (IP) (23), Immunhistochemie (IHC) (21), Radioimmunoassay (RIA) (19), ELISA (Detektionsantikörper) (5), Immunelektrophorese (IEP) (5), Lateral Flow Messung (LF) (5), ELISA (Fangantikörper) (4), Immunodiffusion (ID) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (4), Immunzytochemie (ICC) (3), Immunoelectron Microscopy (IEM) (2), Radiale Immunodiffusion (RID) (2), Konjugation (1), ELISA (Beschichtung) (1), Gel Diffusion (GD) (1), Indirektes ELISA (iELISA) (1)
|
| Produktnummer |
ABIN452559 |
| Menge |
0.5 mg |
| Preis |
440,00 € Zzgl. Versandkosten €20,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
|
Produktmerkmale
|
Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP
|
|
Gen-ID
|
174
|
|
Swiss-Prot
|
P02771
|
|
Immunogen
|
Human AFP, which was isolated from amniotic fluid and purified by AffinityChromatography.
|
|
Kreuzreaktivität
|
Human
|
|
Isotyp
|
IgG2b (Passende Sekundärantikörper)
|
|
Klon
|
AFP-Y1
|
|
Beschreibung
|
Alpha Fetoprotein is a major plasma protein produced by the yolk sac and the liver duringfetal life. Alpha fetoprotein expression in adults is often associated with hepatoma orteratoma. However, hereditary persistance of alpha-fetoprotein may also be found inindividuals with no obvious pathology. The protein is thought to be the fetal counterpart ofserum albumin, and the alpha fetoprotein and albumin genes are present in tandem in thesame transcriptional orientation on chromosome 4. Alpha fetoprotein is found inmonomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids andbilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss ofprotein to screen for spina bifida and anencephaly. Expression has been documented inhuman adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normalhuman brain, liver/spleen, embryo and uterus tissue libraries.
|
|
Spezifität
|
This antibody reacts with Human AFP. No detectable cross-reaction with Human normal serum proteins. Species Reactivity: Tested: Human.
|
|
Applikationshinweise
|
Western blot. Radioimmunoassay. ELISA: This monoclonal AFP antibody can be used as a Capture antibody in a SandwichELISA for Human AFP Detection in combination with a HRP labeled tracer antibody. Suggested Capture Coating Dose: 0.1 µg/well. Substrate: TMBPurification of natural Human AFP. Immunoprecipitation (Qualitative and quantitative).
|
|
Reinigung
|
Aff - Purified
|
|
Buffer
|
0.01M PBS, pH 7.0 without preservatives.Reconstitution: Restore with Double distillated water to adjust the final concentration to1.0 mg/ml.
|
|
Lagerung
|
Store the antibody at -20°C. After reconstitution, aliquot and store at -20°C. Shelf life: one year from despatch.
|
|
Forschungsgebiet
|
Serum/Plasma Proteine
|
|
Beschränkungen
|
Nur für Forschungszwecke einsetzbar
|
Alternativen zu Antigen "alpha-Fetoprotein (AFP)", Typ "Antikörper" finden
|
Wirte
|
Maus (168), Kaninchen (97), Ziege (15), Schaf (3), Huhn (2), Ente (2)
|
|
Reaktivitäten
|
Human (231), Maus (48), Ratte (Rattus) (34), Hund (30), Schwein (26), Rind (Kuh) (23), Kaninchen (20), Pferd (17), Huhn (5), Schaf (4), Affe (3), Fruchtfliege (Drosophila melanogaster) (1), Säugetiere (1), Zebrafisch (Brachydanio rerio) (1)
|
|
Applikationen
|
Western Blot (WB) (95), Enzyme Immunoassay (EIA) (68), Immunfluoreszenz (IF) (68), Durchflusszytometrie (FACS) (65), ELISA (57), Immunhistochemie (Paraffinschnitte) (IHC (p)) (53), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (32), Immunpräzipitation (IP) (23), Immunhistochemie (IHC) (21), Radioimmunoassay (RIA) (19), ELISA (Detektionsantikörper) (5), Immunelektrophorese (IEP) (5), Lateral Flow Messung (LF) (5), ELISA (Fangantikörper) (4), Immunodiffusion (ID) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (4), Immunzytochemie (ICC) (3), Immunoelectron Microscopy (IEM) (2), Radiale Immunodiffusion (RID) (2), Konjugation (1), ELISA (Beschichtung) (1), Gel Diffusion (GD) (1), Indirektes ELISA (iELISA) (1)
|
|
Konjugate
|
HRP (7), Biotin (6), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 647 (4), FITC (4), PE,Cy5.5 (4), Alexa Fluor 555 (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
|
|
Epitope
|
N-Term (4), a (2), C-Term (1), Center (1), b (1), c (1)
|
