TSPO Antikörper (C-Term)

Produktnummer ABIN452349

Der Ziege Polyklonal anti-TSPO Antikörper (ABIN452349) detektiert spezifisch TSPO in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TSPO Antikörper (C-Term) (ABIN452349)

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TSPO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TSPO Antikörper

Bindungsspezifität: C-Term

Sequenz: c-RDNHGWRGGR RLPE

Spezifität: This antibody is expected to recognise one isoform of TSPO/PBR (NP_000705.2) only.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_000705.2. Genename: TSPO

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.1-0.3 μg/mL. Approx 35 kDa band observed in Human Brain lysates(calculated MW of 18.5 kDa according to NP_000705.2). Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Colon shows strongcytoplasm staining of absorptive cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Andere Bezeichnung: TSPO / BZRP

Hintergrund: PBR (Peripheral type Benzodiazepine Receptor) is a ubiquitous protein (18 kDa) found in most steroidogenic tissues. It is located primarily in the outer mitochondrial membrane of steroid producing tissues and is found in association with a 34 kDa voltage dependent anion channel protein (VDAC). PBR interacts with some benzodiazepines and has different affinities than its endogenous counterpart. PBR appears to be a key factor in the transport of cholesterol from the outer to the inner mitochondrial membrane to permit the initiation of steroid hormone synthesis. It is thought to be part of the mitochondrial permeability transition pore (PT) and to be involved in apoptosis. It is speculated that patients with congenital lipoid adrenal hyperplasia, who cannot make any steroids, might have a genetic lesion in BZRP. A short form, PBRS is also expressed in the same tissues, but at a level about ten times that of PBR.Synonyms: MBR, Mitochondrial benzodiazepine receptor, PBR, PKBS, Peripheral-type benzodiazepine receptor, Translocator protein

Gen-ID: 706

NCBI Accession: NP_000705

UniProt: P30536

Pathways: Cellular Response to Molecule of Bacterial Origin