Pancreatic and Duodenal Homeobox 1 (PDX1) Antikörper

Antigen: Pancreatic and Duodenal Homeobox 1 (PDX1)

Synonyme: GSF, IPF1, IUF1, IDX-1, MODY4, PDX-1, STF-1, PDX1, ipf1

Klonalität: Monoklonal (2A12)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), Immunfluoreszenz (IF)

Produktnummer: ABIN411244

Produktbeschreibung

Gen-ID: 3651

Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-283 of human Pdx1

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 2A12

Molekulargewicht: 30.6 kDa

Anwendungen

Applikationshinweise: Western blot: 1:20000, IHC 1:50, IF (1:100)

Konzentration: 0.5-1.0 mg/ml (Lot dependent)

Reinheit: Purified

Reinigung: Purified from mouse ascites fluids by affinity chromatography

Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Forschungsgebiet: Organogenese, Artherosklerose, Abstammungsmarker, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical staining of paraffin-embedded pancreas using anti-PDX1 (ABIN411244) mouse monoclonal antibody. Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-PDX1(ABIN411244) mouse monoclonal antibody. IF Image Anti-PDX1 mouse monoclonal antibody (ABIN411244) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY PDX1(RC222354)

anti-Pancreatic and Duodenal Homeobox 1 (PDX1) antibody (Image 2)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1(RC222354) (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1. Pdx1 antibody (2A12) at 1:20000 dilution + lysate from 293T cells transfected with human Pdx1 expression vector IHC Image Immunohistochemical staining of paraffin-embedded pancreas using anti-PDX1 (ABIN411244) mouse monoclonal antibody.