CA1 Antikörper (Internal Region)

Produktnummer ABIN401513

Der Ziege Polyklonal anti-CA1 Antikörper wird verwendet zum Nachweis von CA1 in Proben von Human, Ratte und Maus. Er wurde validiert für WB und EIA.

Kurzübersicht für CA1 Antikörper (Internal Region) (ABIN401513)

Target: CA1 (Carbonic Anhydrase I (CA1))

Reaktivität: Human, Ratte, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CA1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: c-QAIKTKGKRA P

Spezifität: This antibody detects internal Carbonic anhydrase 1.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, RatSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_001729.1 . Genename: CA1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: 0.03-0.1 μg/mL. Approx 29 kDa band observed in Human Liver lysates(calculated MW of 28.9 kDa according to NP_001729.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu CA1

Target: CA1 (Carbonic Anhydrase I (CA1))

Andere Bezeichnung: Carbonic Anhydrase 1

Hintergrund: Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.Synonyms: CA-I, CA1, CAB, Carbonate dehydratase I, Carbonic anhydrase B, Carbonic anhydrase I

Gen-ID: 759

NCBI Accession: NP_001729

UniProt: P00915