FITC Antikörper

Produktnummer ABIN401128

Dieser Ziege Polyklonal Antikörper detektiert spezifisch FITC in WB, IHC (fro) und EIA.

Kurzübersicht für FITC Antikörper (ABIN401128)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FITC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-FITC Antikörper

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Fluorescein conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/5,000-1/30,000. Western Blot: 1/500-1/3,000. Immunohistochemistry: 1/250-1/1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Andere Bezeichnung: FITC

Substanzklasse: Chemical

Hintergrund: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate