L1/ORF2 Antikörper

Antigen: L1/ORF2

Klonalität: Polyklonal

Wirt: Huhn

Reaktivität: Schimpanse

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN401096

Produktbeschreibung

Produktmerkmale: Synonyms: LINE-1

Weitere Bezeichnung: L1 / ORF2

Immunogen: Synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptidescorrespond to regions within the endonuclease domain of L1/ORF2 protein

Kreuzreaktivität: Schimpanse, Human

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most commonopen reading frame in the human genome, present in various forms in many thousands ofcopies. This large family of proteins includes magnesium dependent endonucleases and alarge number of phosphatases involved in intracellular signaling. Both intact ORF1 andORF2 are absolutely required for autonomous retrotransposition. ORF2 encodes anendonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2appears to be tightly regulated except in germ line tissues, embryonic tissues, and certaincancers including teratomas, testicular cancers, and leukemias. This antibody is intendedfor use in studying control of L1 retrotransposons.

Spezifität: This antibody reacts to L1 / ORF2. Species: Human, Chimpanzee. Others not tested.

Anwendungen

Applikationshinweise: ELISA: 1/10000. Western Blot: 1/2000 - 1/10000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Reinigung: Ig Fraction

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide as preservative

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch

Beschränkungen: Nur für Forschungszwecke einsetzbar