Protein G Antikörper (Texas Red (TR))
Kurzübersicht für Protein G Antikörper (Texas Red (TR)) (ABIN401072)
Target
Alle Protein G Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Produktmerkmale
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Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 2.4 moles Texas Red(TM) per mole of Rabbit IgG -
Aufreinigung
- Ion exchange chromatography
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Immunogen
- Protein G [Streptococcus species]
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Isotyp
- IgG
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Applikationshinweise
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Immunofluorescence. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Rekonstitution
- Restore with 2.0 mL of deionized water (or equivalent). For extended storage reconstitute product with 50 % glycerol.
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Konzentration
- 10.0 mg/mL (by UV absorbance at 280 nm)
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Protein G
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Hintergrund
- Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Target
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