S1-Tag Antikörper

Antigen: S1-Tag

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN398482

Produktbeschreibung

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: S1 tag is an epitope tag composed of a nine-residue peptide, NANNPDWDF, derived from the hepatitis B virus preS1 region. Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems.The Rabbit Anti-S1-tag polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml.

Spezifität: This Antibody recognizes both N-terminal and C-terminal S1 tagged fusion proteins.

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.1-1.0 ug/mlWestern blot using ONE-HOUR WesternTM Kit:For quick results, Antibodies-Online ONE-HOUR WesternTM Kit L00204C is recommended. 20 ug of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized

Konzentration: 1 mg/ml in PBS, pH7.4, containing 0.02% sodium azide

Reinigung: Immunoaffinity chromatography

Lagerung: Rabbit Anti-S1-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-S1-Tag antibody

Western blot analysis of S1 tagged fusion proteins expressed in E. coli cell lysate using S1-tag Antibody (Antibodies-Online, ABIN398482, 1 ug/ml)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.