Mitogen-Activated Protein Kinase Kinase Kinase 10 (MAP3K10) (C-Term) Antikörper

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase 10 (MAP3K10)

Synonyme: MST, MLK2, MEKK10, Mlk2, BC028668, BC046514, mlk2, xmlk2

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN392424

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: MLK2 (C-term)

Gen-ID: 4294

Swiss-Prot: Q02779

Immunogen: This MLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636~666 amino acids from the C-terminal region of human MLK2.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Mixed lineage kinase 2, Mitogen-activated protein kinase kinase kinase 10, Protein kinase MST

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MLK2.

Molekulargewicht: 103623 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Apoptose/Nekrose, Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Zellzyklus, Neurologie, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

The anti-MLK2 Pab (ABIN392424) is used in Western blot to detect MLK2 in transfected COS7 cell lysate. Data is kindly provided by Drs. M.K. Barthwal and A. Rana from Texas A&M University, Health Science Center (Temple, TX).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publikationen

Publikationen: Humphrey, Newcomb, Yu et al.: "Mixed-Lineage kinase-3 stabilizes and functionally cooperates with tribbles 3 to compromise mitochondrial integrity in cytokine-induced death of pancreatic beta cells." in: The Journal of biological chemistry, 2010 (PubMed).