Homeodomain Interacting Protein Kinase 3 (HIPK3) (C-Term) Antikörper

Antigen: Homeodomain Interacting Protein Kinase 3 (HIPK3)

Synonyme: PKY, YAK1, DYRK6, FIST3, ANPK, HIPK3, pky, yak1, dyrk6, fist3, MGC145460

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391779

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: HIPK3 (C-term)

Gen-ID: 10114

Swiss-Prot: Q9H422

Immunogen: This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134~1163 amino acids from the sequence of human HIPK3 within a 1100-1200.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Homeodomain-interacting protein kinase 3, Homolog of protein kinase YAK1, Fas-interacting serine/threonine-protein kinase, FIST, Androgen receptor-interacting nuclear protein kinase, ANPK, DYRK6, FIST3, PKY

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the sequence of human HIPK3 within a 1100-1200.

Molekulargewicht: 133743 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western Blot Immunohistochemistry~~1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Apoptose/Nekrose, Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Zellstruktur, Zellzyklus, Transkriptionsfaktoren, Signaltransduktion, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and Flag-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-Flag antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and Flag-sHIPK3.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publikationen

Publikationen: Tiefenbach, Moll, Nelson et al.: "A live zebrafish-based screening system for human nuclear receptor ligand and cofactor discovery." in: PLoS ONE, Vol. 5, Issue 3, pp. e9797, 2010 (PubMed).