Doublecortin-Like Kinase 1 (DCLK1) (C-Term) Antikörper

Antigen: Doublecortin-Like Kinase 1 (DCLK1)

Synonyme: DCLK, DCDC3A, DCAMKL1, KIAA0369, Ania4, Cpg16, Dcamkl1, DCLK1, dcamkl1, MGC153709, wu:fc51f11, zgc:153709, MGC152477, MGC145348, Dcl, Dclk, Click-I, AI836758, mKIAA0369, 1700113D08Rik, 2810480F11Rik

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), Immunfluoreszenz (IF), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391324

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: DCAMKL1 (C-term)

Gen-ID: 9201

Swiss-Prot: O15075

Immunogen: This DCAMKL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 689~718 amino acids from the C-terminal region of human DCAMKL1.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Serine/threonine-protein kinase DCAMKL1, Doublecortin-like and CAM kinase-like 1

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DCAMKL1.

Molekulargewicht: 82,224 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:10~50 immunofluorescence

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Neurologie, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

The DCAMKL1 Antibody (C-term )(ABIN391324) western blot analysis in mouse heart tissuelysates (35ug/lane).This demonstrates the DCAMKL1 antibody detected the DCAMKL1 protein (arrow).

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DCAMKL1 Antibody (C-term) (ABIN391324), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Expression pattern of doublecortin and CaM kinase-like-1 (DCAMKL-1) in normal mouse small intestine. (A): Immunohistochemical staining of normal small intestine for DCAMKL-1. Arrows indicate the cells positive for DCAMKL-1 in the stem cell zone. (B): Preincubation with blocking peptide completely abolished DCAMKL-1 immunoreactivity. (C): Immunohistochemical staining of normal small intestine for DCAMKL-1. Brown indicates the cells positive for DCAMKL-1 (indicated by the arrows).

Publikationen

Publikationen: Gerbe, Brulin, Makrini et al.: "DCAMKL-1 expression identifies Tuft cells rather than stem cells in the adult mouse intestinal epithelium." in: Gastroenterology, Vol. 137, Issue 6, pp. 2179-80; author reply 2180-1, 2009 (PubMed).