Amylo-alpha-1, 6-Glucosidase, 4-alpha-Glucanotransferase (AGL) (C-Term) Antikörper

Antigen: Amylo-alpha-1, 6-Glucosidase, 4-alpha-Glucanotransferase (AGL)

Synonyme: GDE, C77197, AI850929, 1110061O17Rik, 9430004C13Rik, 9630046L06Rik

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunfluoreszenz (IF), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN389030

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: AGL (C-term)

Gen-ID: 178

Swiss-Prot: P35573

Immunogen: This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1487~1516 amino acids from the C-terminal region of human AGL.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1,4-1,4-glucantransferase, Amylo-alpha-1,6-glucosidase, Amylo-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, GDE

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AGL.

Molekulargewicht: 174764 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western Blot~~1:100-500 Immunofluorescence

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Proteinmodifikationen, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot using anti-AGL (C-term) antibody (ABIN389030) at 1:500 dilution. A total of 20 ug of lysates was loaded for each tissue. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan.

Expression of myc-GS causes wild type but not the �CBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL �CBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.

Publikationen

Publikationen: Parker, Kong, Walsh et al.: "Fast-twitch sarcomeric and glycolytic enzyme protein loss in inclusion body myositis." in: Muscle & nerve, Vol. 39, Issue 6, pp. 739-53, 2009 (PubMed).