SMURF2 Antikörper (C-Term)

Produktnummer ABIN388831

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SMURF2 in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Ratte.Es wurde in 1 Publikation zitiert.

Kurzübersicht für SMURF2 Antikörper (C-Term) (ABIN388831)

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMURF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB4107-4108

Produktdetails anti-SMURF2 Antikörper

Bindungsspezifität: AA 702-731, C-Term

Homologie: M, X

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 702-731 amino acids from the C-terminal region of human SMURF2.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:10~50. IF: 1:10~50. WB: 1:2000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-SMURF2 Antikörper (ABIN388831)

Schwamborn, Khazaei, Püschel: "The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The Journal of biological chemistry, Vol. 282, Issue 48, pp. 35259-68, (2007) (PubMed).

Details zu SMURF2

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Andere Bezeichnung: SMURF2

Hintergrund: SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with SMAD1, SMAD2 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. It enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Molekulargewicht: 86196

Gen-ID: 64750

NCBI Accession: NP_073576

UniProt: Q9HAU4