THETM c-Myc Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates.
Produktmerkmale
This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/mL Western blot: 0.1-1 µg/mL Other applications: user-optimized
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freezing and thawing cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Armstrong, Xiao, Krill, Seroude, Dawson-Scully, Robertson: "Glial Hsp70 protects K+ homeostasis in the Drosophila brain during repetitive anoxic depolarization." in: PLoS ONE, Vol. 6, Issue 12, pp. e28994, (2011) (PubMed).