Maus anti-Kaninchen IgG Antikörper (PE)

Antigen: IgG

Klonalität: Monoklonal (2A9)

Wirt: Maus

Reaktivität: Kaninchen

Konjugat: PE

Applikation: ELISA, Durchflusszytometrie (FACS)

Produktnummer: ABIN376541

Produktbeschreibung

Isotyp: IgG1

Klon: 2A9

Beschreibung: WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Spezifität: Reacts with the heavy chain of all subclasses of rabbit IgG as demonstrated by ELISA, may also react with IgG from other species.

Anwendungen

Applikationshinweise: Flow Cytometry: Fluorescein conjugate ≤ 3 µg/106 cells R-phycoerythrin conjugate ≤ 0.3 µg/106 cells Spectral Red™ conjugate ≤ 0.3 µg/106 cells ELISA: Purified antibody 1:4,000-1:8,000 AP conjugate 1:2,000-1:4,000 HRP conjugate 1:4,000-1:8,000 BIOT conjugate 1:5,000-1:20,000 Other Applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Konzentration: 0.1mg/ml

Lagerung: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8°C. • The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8°C. • The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8°C or long-term at - 20°C. • The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C. • The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8°C. • The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! • The Spectral Red™ (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! • Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

Forschungsgebiet: Immunologie, Sekundärantikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar