GOT2 Antikörper (Internal Region)

Produktnummer ABIN374942

Der Ziege Polyklonal anti-GOT2 Antikörper wird verwendet zum Nachweis von GOT2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für GOT2 Antikörper (Internal Region) (ABIN374942)

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GOT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GOT2 Antikörper

Bindungsspezifität: Internal Region

Spezifität: This antibody reacts to GOT2.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence CKDADEAKRVES, from the internal region of the protein sequence

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/32000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GOT2

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Andere Bezeichnung: GOT2

Hintergrund: GOT2 [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80 % of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.Synonyms: Aspartate aminotransferase, FABP-1, FABPpm, Fatty acid-binding protein, Transaminase A, mAspAT, mitochondrial

Gen-ID: 2806, 9606

UniProt: P00505

Pathways: Monocarboxylic Acid Catabolic Process