Glucose-6-Phosphate Dehydrogenase Antikörper (Internal Region)

Produktnummer ABIN374790

Der Ziege Polyklonal anti-Glucose-6-Phosphate Dehydrogenase Antikörper (ABIN374790) detektiert spezifisch Glucose-6-Phosphate Dehydrogenase in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Hund.

Kurzübersicht für Glucose-6-Phosphate Dehydrogenase Antikörper (Internal Region) (ABIN374790)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glucose-6-Phosphate Dehydrogenase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-Glucose-6-Phosphate Dehydrogenase Antikörper

Bindungsspezifität: AA 308-320, Internal Region

Sequenz: C-STNSDDVRDE KVK

Spezifität: This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Dog (Canine).
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.

Immunogen: Synthetic Peptide from the internal region (aa 308-320) of Human G6PD. Genename: G6PD

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution: 1/32000. Western Blot: Use at 0.03-0.1 μg/mL. Detects a double band of approx. 55-60 kDa band in HumanTestis, Thyroid and Tonsil lysates. Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Testis shows strongcytoplasm staining in spermatogonia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month (in aliquots) at -20 °C for longer.

Details zu Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: G6PD

Hintergrund: Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase

Gen-ID: 2539, 9606

UniProt: P11413

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones