DIO2 Antikörper (Internal Region)

Produktnummer ABIN374755

Dieser Ziege Polyklonal Antikörper detektiert spezifisch DIO2 in IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus und Schwein.

Kurzübersicht für DIO2 Antikörper (Internal Region) (ABIN374755)

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Reaktivität: Human, Ratte, Maus, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DIO2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-DIO2 Antikörper

Bindungsspezifität: Internal Region

Spezifität: This antibody is expected to recognise both reported isoform a and isoform b of DIO2.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Rat and Porcine.
Species reactivity (tested):Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography

Immunogen: Peptide with sequence EVKKHQNQEDRC, from the internal region of the protein sequence

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection limit: 1/128000. Western Blot: Preliminary experiments gave an approx 65-70 kDa band in Human Heart andThyroid lysates after 0.05 μg/mL DIO2 antibody staining. Immunohistochemistry: 2-3 μg/mL. Cryo-sectioned Mouse Brain (Hippocampus) showsstaining in the granular layer of the dentate gyrus.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu DIO2

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Andere Bezeichnung: DIO2

Hintergrund: DIO2 belongs to the iodothyronine deiodinase family and is responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). It is essential for providing the brain with appropriate levels of T3 during the critical period of development. DIO2 is expressed in heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. There are two named isoforms.Synonyms: DIOII, ITDI2, TXDI2, Type II iodothyronine deiodinase, Type-II 5'-deiodinase

Gen-ID: 1734

NCBI Accession: NP_000784

UniProt: Q92813

Pathways: Hormone Transport, Hormone Activity