PVALB Antikörper (C-Term)

Produktnummer ABIN374572

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PVALB in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für PVALB Antikörper (C-Term) (ABIN374572)

Target: PVALB (Parvalbumin (PVALB))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PVALB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PVALB Antikörper

Bindungsspezifität: C-Term

Sequenz: C-GVDEFSTLVA ES

Spezifität: This antibody recognizes Parvalbumin.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_002845.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx 12 kDa band observed in Human Brain lysates (calculated MW of 12.2 kDa accordingto NP_002845.1). Approx 10-12 kDa band observed in rat skeletal muscle and in rat heart cells transfectedwith human PVALB, but not in untransfected rat heart cells. (1)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PVALB

Target: PVALB (Parvalbumin (PVALB))

Andere Bezeichnung: Parvalbumin alpha / PVALB

Hintergrund: Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.

Gen-ID: 5816

NCBI Accession: NP_002845

UniProt: P20472